Global Expression Analysis

You can search for global expression by:

- which proteins you are interested in
- the direction of differential expression: over, under, none or etc
- which tissue of origin

tips:
- leaving the protein selection blank will apply your condition query to all reported proteins
- tissue of origin box will rank the proteins found for differential expression across all organisms in the database.

e.g., to search for all proteins that are differentially expressed in cns, colon, leukemia and melanoma, leave the protein box blank, make sure that only these origins are marked then click search.

If your query matches more than one protein then the results will be displayed in a heatmap. Each row of the result table corresponds to one protein's differential expression. The complete description of all displayed fields is as follows:

Protein: These are clickable entries and take you to a one page summary of information about that protein.
Gene name: Gene name of the corresponding protein.
Organs: The text above the heatmap shows the tissue of origin in which the protein is significantly differentially expressed.
Heatmap cells: Each heatmap cell displays the number of studies where a protein is observed significantly over or under expressed.

Clicking on the cell will show more information about the expression of that protein. The heatmap cell color can be blue- for over, or gray- for under expression.
Cells that are half blue and half gray indicate that there are experiments that show both over and under expression of the protein.

Need help?

Search for proteins by protein name, gene name, gene ontology, protein description or IPI identifier. After typing at least 3 letters a list of possible queries will pop up. You can combine as many keywords as you want.

The color-coding has the following scheme:

Protein name:

14-3-3 protein epsilon

Gene name:

YWHAE

Gene ontology (GO):

GO:0021987

Protein description:

14-3-3 PROTEIN EPSILON.

IPI identifier:

IPI00000816

Search for

Specifies the direction of differential expression. For example we ask whether each protein expression level in each organ is significantly different from the mean expression for all organs. To read more about the statistical methodology, please see the publication.

Expression

Select tissues which are considered in the expression analysis. The mean protein intensities of the selected tissues are compared to the mean intensity of the selected reference (Ref), or in case of Average to the mean protein intensity over all selected tissues.

Tissue of Origin

List: Flat table of the expression analysis, where each protein is (at most) represented once per selected tissue.
Heatmap: Table in a heatmap syle, where proteins are listed row-wise and selected tissues column-wise.

View